To confirm the finding from the discovery PheWAS, we conducted an additional analysis in the LLFS cohort, where we observed a replication of the negative association between CYP2A6 rs113288603 and hearing loss symptoms in nicotine-exposed subjects (p = 0.098), and as with the discovery sample, no association was observed in the LLFS nicotine-unexposed sample (p = 0.524). The gene discussed is CYP2A6; the disease is hearing loss disorder.