To determine the role of PRDX2 in colon cancer cells treated with 5-FU, we used the CCK-8 assay to detect the survival rate of colon cancer cells that were stably transfected with NC-shRNA-LV and PRDX2-shRNA-LV and treated with 5-FU at different concentrations for 48 h. This evidence concerns the gene PRDX2 and malignant colon neoplasm.