In patients with TCC/PCA, S100A8/A9USG, S100A8/A9Cre, S100A8/A9Prot, S100A12USG, S100A12Cre, S100A12Prot, and uCalR did not differ between dogs with a single tumor site (bladder, urethra, or prostate; n = 53) and dogs with more than one of those sites affected (n = 9; all P > 0.05). This evidence concerns the gene S100A8 and tarsal-carpal coalition syndrome.