RAD50 and leukemia: Since this pathway was shown to operate in myeloid cells [42], we stably infected a human leukemia monocytic cell line (THP-1) with a recombinant KSHV virus (TrK.219, see Materials and methods) and used it to confirm the interaction between LANA and the Rad50-Mre11-CARD9 DNA sensor complex (Fig 2C).