In our study, we also identified six genes (ABAT, AGXT, ALDH6A1, CYP4A11, DAO and EHHADH) in association with the high risk in metastasis prediction, and none of the genes were included in the 161-gene set of Roessler et al. In further analyses, these six genes were also significantly associated with HCC prognosis including recurrence and survival. The gene discussed is ALDH6A1; the disease is hepatocellular carcinoma.