To determine whether up-regulating JMJD3 in healthy T cells was sufficient to induce B cell overstimulation, resembling that of lupus T cells, CD4+ T cells expressing pcDNA3.1-JMJD3 were cultured with purified autologous B cells (1:4 ratio) for 8 days, and IgG production was then measured using enzyme-linked immunosorbent assays (ELISAs). The gene discussed is CD4; the disease is systemic lupus erythematosus.