Moreover, BDNF enhanced midkine-mediated anoikis resistance, while treatment with the TrkB inhibitor K252a or TrkB-specific siRNA made HCC cells sensitive to anoikis mediated by both midkine and BDNF in the presence of midkine and also led to a slight decrease in the expressions of pAkt. This evidence concerns the gene BDNF and hepatocellular carcinoma.