Other examples of this complexity include SPRY4-IT1, which is derived from an intron of SPRY4, is upregulated in many cancers and has been shown to induce EMT in colorectal cancer, gliomas, HCC, oesophageal squamous cell carcinoma and osteosarcoma [264,266,267,380,381,382,383,384]. This evidence concerns the gene SPRY4 and osteosarcoma.