In the same validation analysis, we measured another chaperone protein, HSC70, and TDP-43, both not differential in the 2D-DIGE analysis, but up-regulated in PBMC of a cohort of ALS patients with a mean age of onset of 62 ± 10 years, previously reported in a work by our laboratory (Nardo et al., 2011). Here, HSPA8 is linked to amyotrophic lateral sclerosis.