For NLRP3 inflammasome activation, the expression of IL-1β, IL-18, the NLRP3 inflammasome and ACS as well as caspase-1 separated from pro-caspase-1 in KCs with knock-down of Foxo3a were all significantly higher than in those KCs treated with PA and LPS alone (Supplementary Figure 4B and 4C). This evidence concerns the gene FOXO3 and dry eye syndrome.