For NLRP3 inflammasome activation, the expression of IL-1β, IL-18, the NLRP3 inflammasome and ACS as well as caspase-1 separated from pro-caspase-1 in KCs with knock-down of Foxo3a were all significantly higher than in those KCs treated with PA and LPS alone (Supplementary Figure 4B and 4C). The gene discussed is IL18; the disease is dry eye syndrome.