Here, we observed that the primary prerequisite for the activation of TLR3-mediated apoptosis pathway is (i) low-to-medium levels of TLR3 protein in the cancer cell lines and (ii) sufficient engagement of polyI:C and TLR3 for activation of TLR3 signalling, where cell death ensues regardless of the aggressiveness of the cancer cell type. Here, TLR3 is linked to cancer.