Finally, in order to further validate these 5 DEGs, RT-qPCR were performed and the result was the same with that of RNA-seq, demonstrating that CCL3, CCL3L1, JUN, IL8, and IL1B were reliable and available to be used as the candidates for hepatic carcinoma with metastasis (Figure 6A and 6B). Here, IL1B is linked to hepatocellular carcinoma.