Finally, in order to further validate these 5 DEGs, RT-qPCR were performed and the result was the same with that of RNA-seq, demonstrating that CCL3, CCL3L1, JUN, IL8, and IL1B were reliable and available to be used as the candidates for hepatic carcinoma with metastasis (Figure 6A and 6B). The gene discussed is CCL3L3; the disease is hepatocellular carcinoma.