The control-treated cells displayed expected clonogenic activity in both 2D assays (Figures 3C, 3D) and 3D assays (Supplementary Figures 2A and S2B), whereas the BRCA1-shRNA-transduction produced a pronounced loss of clonogenic activity in both the BC and LP fractions (Figure 3D) with no significant induction of multipolar spindle architecture (Supplementary Figure 2C). The gene discussed is BRCA1; the disease is breast cancer.