For example, we were only capable of examining two cytokines using a single parameter, whereas use of multi-parametric flow cytometry would have allowed for additional insight into the characteristics of CD4+ T-cell or CD8+ T cell populations and their role in malaria-induced pathology e.g., IFN-γ/IL-10 and IFN-γ/IL-17 co-producing cells, etc. In addition, we did not measure the levels of IL-27 that has been shown to suppress IFN-γ production by CD4+ T-cells during malaria infection [80]. The gene discussed is CD8A; the disease is malaria.