An ANO1 protein, with a molecular weight of ~114 kDa was detected by Western blot, suggesting that the protein was most likely translated from E1 (ANO-941) and/or E2 variants (ANO1-790 and ANO1-627) based on expression levels in both ICC (Fig 6C) as well as predicted molecular weight (Fig 7A and 7C): 116.81 kDa for ANO-941, 113.41 kDa for ANO1-790, and 110.93 kDa for ANO1-627 [28]. Here, ANO1 is linked to intrahepatic cholangiocarcinoma.