The expression levels of several cell-specific markers for cells found in GI tissue (Kit for ICC, Myh11 for SMC, Pdgfra for PDGFRα+ cells, and Uchl1 [aka PGP9.5] for neuronal cells) were quantified to further validate the identity of the ICC obtained after FACS. The gene discussed is KIT; the disease is intrahepatic cholangiocarcinoma.