However, other Cl- channel subunits such as Fxyd1, Clic1, Clcn4-2 and Clic4 were also expressed in ICC, but at much lower levels than Ano1. Analysis of Ano1 within the UCSC Smooth Muscle Genome Browser showed that Ano1 was transcribed into as many as ten different transcriptional variants in ICC of both organs (Fig 6A). This evidence concerns the gene CLIC4 and intrahepatic cholangiocarcinoma.