We next used recombinant NKG2D-Fc fusion protein to detect binding capacity of ovarian cancer cell surface NKG2DL, and found the positive NKG2D staining rates in OVCAR3, SKOV3, A2780, HO8910 and 3AO cells were 8.2%, 10.5%, 42.1%, 67.8% and 75.1%, respectively (Fig 5). The gene discussed is KLRK1; the disease is ovarian cancer.