To confirm that TBK1 is involved in RGC senescence caused by acute IOP elevation-induced retinal ischemia, the TBK1 inhibitor BX-795 was injected into the vitreous in concentrations of 50 nM, 500 nM or 5 μM. We performed western blot analysis to determine protein levels of TBK1, p16 and cyclin A, as well as SA-β-gal staining of frozen retinal sections and retinal flats. This evidence concerns the gene TBK1 and retinal ischemia.