Proteins of interest were identified in both fractions, HAP and LAP, we focused on 4 proteins(Fig. 4A), two of them located in a cluster that included diabetes related proteins: 14-3-3 protein beta/alpha(spots 22 and 24 from HAP) and Zinc-alpha-2-glycoprotein(ZAG; spots, 26, 36, and 37 from LAP); one protein included in clusters with tendencies related to diabetes and obesity, Beta-2-glycoprotein or ApoH(spots 40, 47, and 51 from LAP), and Otopetrin 3(OTOP3, spot 37 from HAP) that showed an obesity-related behaviour. The gene discussed is APOH; the disease is obesity due to melanocortin 4 receptor deficiency.