We found the highest expression of chemokines and cytokines in BCG-treated melanoma skin metastases 4 weeks after IL-BCG injection are CCL2 (40.8 vs. 157.1, of the average FPKM in BCG uninjected vs. BCG injected melanoma tissues), CCL3 (12.4 vs. 75.7), CCL4 (13.1 vs. 109.9), CCL5 (26.7 vs. 73.3), CCL18 (54.5 vs. 520), CXCL9 (48.8 vs. 475.3), and CXCL10 (41.6 vs. 404.3) (Table 1). This evidence concerns the gene CXCL9 and melanoma.