Decreased levels of Th1-polarizing Flt3L and IL-15 chemokines and decreased levels of DC-recruiting chemokines (29), MIP-1α and MIP-1β, were observed in the conditioned media of monocytes matured with melanoma EVs, suggesting in addition to compromising maturation, EV-conditioned DCs may elicit functional effects on immune cells downstream and modulate DC migration. This evidence concerns the gene CCL4 and melanoma.