In this study, we identified melanoma-derived EVs containing cargo enriched for immune regulatory proteins including S100A8, S100A9, Annexin A1, and ICAM1 (Table 1) and further demonstrated that all of the proteins as well as S100A8 or S100A9 individually have the functional capacity to compromise DC maturation (Figure 4). The gene discussed is S100A9; the disease is melanoma.