Similarly, immunohistochemistry analysis has revealed specific staining for CD4, CXCR5, and ICOS on infiltrating immune cells in the synovial tissues of RA patients, and the presence of Tfh cells (CD4+CXCR5+ICOS+ T cells) in the synovial tissues of RA patients has been verified using both triple-fluorescence immunostaining and confocal laser scanning (82). This evidence concerns the gene CD4 and rheumatoid arthritis.