TLR4 and rheumatoid arthritis: To examine whether TLR4 protein is as well expressed on the surface of CD8+ T cells we analyzed TLR4 expression on CD3+CD8+ T cell population from the whole blood or on isolated CD8+ T cells using RosetteSep CD8+ T cells enrichment kit from RA patients and healthy donors (HC) using flow cytometry (Supplementary Fig. 1a,b).