Shen et al. [27] after treating a co-culture of U87-MG and C6 glioma cell lines in transwell plates with the NO donor sodium nitroprusside (SNP), or NAME (Nω-nitro-l-arginine methyl ester), a NOS inhibitor, recorded respectively the increase and the significant inhibition of tumor cell migration. The gene discussed is NOS2; the disease is central nervous system cancer.