In this analysis, correlations between HDAC1 expression and tumor stage, tumor grade, lymph node metastasis or distant metastasis were observed in gastrointestinal malignancies, indicating that HDAC1 might be a good biomarker to distinguish different stages, grades, and states of lymph node metastasis or distant metastasis and would be beneficial for the diagnosis of gastrointestinal malignancy. The gene discussed is HDAC1; the disease is metastatic malignant neoplasm in the lymph nodes.