With the use of the same hepcidin ELISA kit as the current study (Bachem), we assessed the diagnostic performance of hepcidin as an index of iron deficiency and the need for iron supplementation in West and East African preschool children and found that the AUCROC for hepcidin to identify iron deficiency was 0.85, with a threshold that used the Bachem ELISA of 5.5 ng/mL to distinguish iron deficiency across the overall population and among anemic children (14). This evidence concerns the gene HAMP and Iron deficiency anemia.