To test the utility of the Prdm16cGTinv allele for conditional ablation studies, as well as to explore Prdm16 function in the brain and evaluate these conditional mutants as a model for adult neurological disease, we conditionally removed Prdm16 in the embryonic forebrain by crossing Prdm16cGTinv/cGTinv mice to the Emx1IREScre (Emx1::cre) deleter strain (Gorski et al., 2002). The gene discussed is EMX1; the disease is nervous system disorder.