In this study, we noticed that artocarpin is able to induce apoptosis in not only p53-wild type tumor cells (A549), but also p53-mutant tumor cells (H1299 and H226) (Figure 1A and 1B).Moreover, we found that activation of p53 by artocarpin subsequently regulated downstream apoptotic proteins, including PUMA, Cytochrome C, Apaf-1 and caspase 3, and such effects were ameliorated by reduction of ROS and inhibition of ERK1/2 or p38 MAPK. This evidence concerns the gene APAF1 and neoplasm.