To address the role of tumor-derived MMP9 in tumor vascularization, we utilized MDA-MB-231 cells expressing constitutively-active ALK5/TGFBR1-T204D (caALK5) and MDA-MB-231-caALK5 cells depleted of MMP9 by shRNA [13]. This evidence concerns the gene TGFBR1 and neoplasm.