When we co-expressed YFP-RhoA and RF-actin-BP in NSCLC cells, we noticed that while exposure to the PCAIs did not alter the localization of the YFP-RhoA protein, they decoupled the apparent co-localization of both proteins as depicted by changes in the relative intensities of the fluorescence profiles of YFP-RhoA and RFP-F-actin, with that of RFP-F-actin significantly decreasing at the cell membrane. The gene discussed is RHOA; the disease is non-small cell lung carcinoma.