In contrast to the robust effects of RBF3 on the EGFR+ or HER2+ breast cancer cell lines, RBF3 and DTT did not induce an ER stress response in wild type Mouse Embryo Fibroblasts (MEFs) and weakly suppressed Akt phosphorylation and induced PARP cleavage (Figure 3D). This evidence concerns the gene AKT1 and breast cancer.