To determine if the ROS level in BITC-treated prostate cancer cells is vital for the induced cell death, we detected the cell viability in BITC-treated Rv1 and PC3 cells with or without the pretreatment of 10 mM NAC, which may neutralize BITC in the medium, 5,000 U/ml CAT, which suppressed BITC-induced ROS, and 1 or 10 mM 3-AT, which inhibits endogenous catalase activities. Here, CAT is linked to prostate carcinoma.