Because BITC-mediated ROS generation, MMP disruption, cytochrome c release, caspase 3/7 activation and cytoplasmic histone-associated DNA fragmentation were significantly attenuated in the presence of NAC (Figure 3), it is reasonable to conclude that the death of human prostate cancer cells caused by BITC was triggered by ROS generation. This evidence concerns the gene CYCS and prostate carcinoma.