NKD1 and melanoma: To further validate these results, and since NKG2D engagement by MICA/B not only triggers the cytolytic activity of NK cells, but also their cytokine production, we next evaluated IFN-γ production by NK cells isolated from a healthy donor (Nkd1) co-cultivated with T1 melanoma target cells, pre-treated with CAFs CMs or control medium, in the presence or absence of GM6001.