To verify whether the phosphorylation of Akt in CEF and DF-1 cells was PI3K dependent or independent following infection with NDV, the specific PI3K inhibitors LY294002 (10 and 20 μM) and wortmannin (0.2 and 1 μM) were incubated for 1 h prior to infection with NDV. This evidence concerns the gene AKT1 and infection.