In this work, considering that PUMA is a transcript target of p53/p73, we first selected A2780s, OVCAR-3, SKOV3 and A2780cp ovarian cancer cells as human cell models because these cells have different p53 status, that is, their p53 status are p53 wild-type, p53 mutant, p53−/− and p53 wild-type (loss of p53 function), respectively [2, 12, 27, 28]. Here, BBC3 is linked to ovarian cancer.