Via western blot assay, we found that Notch1 is highly expressed in 5 out of 7 HCC cell lines (high in SMMC-7721, QGY-7703, HTB-52 (Sk-hep1), Huh7 and Bel7402 cells, but rare in Hep3B and HepG2 cells) and in normal liver cells. Here, NOTCH1 is linked to hepatocellular carcinoma.