The present study (1) establishes the antitumor effectiveness of an anti-PD-L1 antibody in different murine s.c. and intravesical bladder tumor models and (2) provides evidence of heightened therapeutic actions of PD-1/PD-L1 blockade when combined with NHS-muIL12, a pro-inflammatory immunocytokine. Here, PDCD1 is linked to urinary bladder neoplasm.