MYC and cancer: GSEA demonstrated that genes downregulated upon FACT knockdown belong to four functional groups, (i) genes involved in regulation of cell cycle and DNA replication, (ii) genes overexpressed in ES cells or in undifferentiated versus differentiated cancers; (iii) genes upregulated by several pro-oncogenic factors, including Myc, EGFR, RalA, RalB, and RhoA, and (iv) genes that are targets of transcriptional factors of the E2F family (Table 1).