We evaluated whether CTGF had an inhibitory effect on COL1A1 expression in tightly compacted spheroids and observed that CTGF knockdown significantly reduced COL1A1 synthesis (Figure 6D), decreasing interaction between HCC cells (Figure 6E) and resulting in greater therapeutic efficacy of anticancer drugs, which are sorafenib and cisplatin, in HCC spheroids (Figure 6F). The gene discussed is CCN2; the disease is hepatocellular carcinoma.