When HCC progresses from fibrosis, the normal structure of liver is lost, with the formation of increasingly dense fibrous septae of fibronectin and collagens I, III, and V. In Figures 3 and 4, we revealed that COL1A1, which is regarded as the most important collagen in fibrosis, is upregulated in all tightly compacted spheroids. Here, FN1 is linked to hepatocellular carcinoma.