When HCC progresses from fibrosis, the normal structure of liver is lost, with the formation of increasingly dense fibrous septae of fibronectin and collagens I, III, and V. In Figures 3 and 4, we revealed that COL1A1, which is regarded as the most important collagen in fibrosis, is upregulated in all tightly compacted spheroids. This evidence concerns the gene COL1A1 and hepatocellular carcinoma.