As the miR-27a was found to be overexpressed in colorectal cancer stem cells, we knockdown the miR-27a by its specific antisense oligonucleotides to investigate the relationship between miR-27a and sensitivity to TRAIL in colorectal cancer (transfection efficiency of miR-27a antioligonucleotides was shown in Figure 2B). The gene discussed is TNFSF10; the disease is colorectal cancer.