In order to evaluate the aggressiveness of the lesions across different tumor types and to further classify HCC variants obtained from hepatocyte and HPC tracing, resected tumors were further analyzed by IHC, a “HAGCKS” (HSP70, AFP, GS, CK19, Ki67, and Sox9) score with arbitrary units was created to classify tumors aggressiveness, as previously reported (Gomes et al., 2016) (Supplemental Experimental Procedures; Figures S5D–S5V). This evidence concerns the gene SOX9 and hepatocellular carcinoma.