KPNA2 expression in four different human hepatocellular carcinoma cell lines, HepG2, SMMC-7721, Hep3B, and Huh-7 cells was further examined using quantitative real-time PCR and it was revealed that Huh-7 cells showed the highest KPNA2 expression, while HepG2 and SMMC-7721 showed similar KPNA2 expression, which were about 1/3 of that in Huh-7 cells (Figure 1B). Here, KPNA2 is linked to hepatocellular carcinoma.