Then, the functional characteristics of these deregulated genes were analyzed using Ingenuity Pathway Analysis (IPA), and it was revealed that several critical pathways crucially involved in cancer development were deregulated by KPNA2 knockdown in HepG2 hepatocellular carcinoma cells, with the cell cycle pathway at the G2/M phase and control of chromosomal replication (S phase), the ATM signaling pathway, and the PLK kinase pathway as the top canonical pathways (Figure 7B). The gene discussed is ATM; the disease is cancer.