KPNA2 and hepatocellular carcinoma: To investigate the impact of KPNA2 knockdown on hepatocellular carcinoma tumorigenesis, a colony formation assay was performed and showed that the colony formation ability was obviously impaired in the hepatocellular carcinoma cell lines HepG2 (Figure 3C) and SMMC-7721 (Figure 3D) infected with KPNA2-shRNA compared with cells infected with Scr-shRNA, both which were cultured for 14 days.