To investigate the molecular functions of KPNA2 in tumorigenesis of human hepatocellular carcinoma, a lentivirus-mediated short-hairpin RNA (shRNA) strategy was chosen to inhibit KPNA2 expression in two human hepatocellular carcinoma cell lines, HepG2 and SMMC-7721. Here, KPNA2 is linked to hepatocellular carcinoma.