The protein level of EGFR, phosphorylated ERK and AKT, MMP-2 was significantly lowered in PC cell lines after transfection of miR-133a/b, which indicate miR-133a/b suppress EGFR in PC cells whereby inactivating the downstream signals, MMP-2, as an effector of EGFR pathway and mediating cell migration and invasion [49], similar mechanisms may occur in NSCLC [51]and in bladder cancer [50]. This evidence concerns the gene MMP2 and pachyonychia congenita.