To confirm the pattern of gene expression of TLR3 and TRIF (TIR Domain-Containing Adapter Protein Inducing IFN-β) an intermediate adapter protein in TLR3 signaling pathway, we performed a quantitative real time PCR with cDNA synthesized from total RNA obtained from control and reovirus (5 MOI) treated HCT116 colorectal cancer cell line at 24 hours. Here, IFNB1 is linked to colorectal cancer.