TLR3 and colorectal cancer: To confirm the pattern of gene expression of TLR3 and TRIF (TIR Domain-Containing Adapter Protein Inducing IFN-β) an intermediate adapter protein in TLR3 signaling pathway, we performed a quantitative real time PCR with cDNA synthesized from total RNA obtained from control and reovirus (5 MOI) treated HCT116 colorectal cancer cell line at 24 hours.