Here, our results demonstrated that the expressions of fibrosis-related cytokines IL-6 and myofibroblast markers COL1A1 were significantly increased in the supernatant of TGF-β1 induced MLF, serum, and BALF in mice with BLM-induced pulmonary fibrosis, especially in BLM 42d group. The gene discussed is COL1A1; the disease is pulmonary fibrosis.