To evaluate whether inhibitory effects of ALS-L1023 on hepatic steatosis in obese mice were associated with alterations of the expression of genes targeted by peroxisome proliferator-activated receptor α (PPARα), we measured mRNA levels of PPARα target genes such as fatty acyl-CoA oxidase (ACOX), carnitine palmitoyltransferase I (CPT-I), medium-chain acyl-CoA dehydrogenase (MCAD), and very long-chain acyl-CoA dehydrogenase (VLCAD), which encode enzymes involved in fatty acid β-oxidation. This evidence concerns the gene ACADM and fatty liver disease.