As expected, expression of GBP-1 was vastly reduced in H5N1 infected JH4 cells overexpressing RIG-I and MAVS with concurrent GBP-1 knockdown at both 12 and 24 hours post infection relative to cells overexpressing RIG-I and MAVS without GBP-1 knockdown (Figure 4B). This evidence concerns the gene RIGI and infection.