To investigate the role of EPOR in tumor development in vivo, MDA-MB-231-D3H2 cells harboring either a tet-on-inducible shEPOR (shEPOR) or a tet-on-inducible scrambled shSCR sequence (shSCR) were prepared and validated prior to subcutaneous (s.c.)injection in B6N nude female mice (Supplementary Figure S4A, S4B, S4C and S4D). This evidence concerns the gene EPOR and neoplasm.