To study whether PI3K/AKT inhibition can suppress esophageal cancer cell motility and reverse the invasiveness of I3 cells, a vector expressing PTEN was transfected into KYSE410-I3 and KYSE510-I3 cells, as well as KYSE270 and T.Tn which were ESCC cell lines with relatively high invasive ability. This evidence concerns the gene PTEN and esophageal squamous cell carcinoma.