The KYSE410-I3 and KYSE510-I3 sublines showed significantly higher invasive potential (Figure 1A), and enhanced EMT as indicated by marked decrease in E-cadherin and increase in N-cadherin expression (Figure 1B), compared with their respective parental ESCC cell lines, although no significant difference in morphology was observed (Figure 1C). This evidence concerns the gene CDH2 and esophageal squamous cell carcinoma.