FLT3 and acute myeloid leukemia: To analyse the effects of FLT3 inhibition on HH signaling downstream targets, the FLT3-mutated AML cell lines MV4-11 and MOLM-13 as well as the FLT3 wildtype AML cell line OCI-AML5 were treated with 50 nM and 100 nM sunitinib, respectively, and western blot analysis for GLI1 as active HH signaling readout was performed (Figure 6).