In order to investigate the potential of a combined inhibition of GLI, FLT3 and PI3K in FLT3-mutated AML in vivo, NSG mice were transplanted with MV4-11 cells and treated with GANT61, sunitinib, PF-04691502, their combination or DMSO as a control. The gene discussed is FLT3; the disease is acute myeloid leukemia.