Further, canonical Wnt transcriptional activity, Axin2 transcript abundance, and E-cadherin reporter activity were minimally changed by niclosamide treatment in Axin2 knockdown colon cancer cells (Figure 4C), indicating that Axin2 is required for mode of action (MoA) of niclosamide on colon cancer cells. Here, AXIN2 is linked to malignant colon neoplasm.